Hieff clone plus multi one step cloning kit

WebDescription. The GenBuilder Plus Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any linearized ... Web11 de nov. de 2024 · The In-Fusion cloning based on in vitro homologous recombination was performed to generate all the constructs using an In-Fusion HD Cloning Kit (Clontech) and Hieff Clone Plus Multi One Step ...

GenBuilderTM Plus Cloning Kit User Manual - GenScript

Web产品描述. Hieff Clone TM Plus Multi One Step Cloning Kit 是一款简便、快速、高效的 DNA 定向克隆产品，该试剂盒可以将 PCR 产物定向克隆至任何载体的任何位点 ，可高效克隆 50 bp-10 kb 片段。 将 载体线性化，并在插入片段正、反向 PCR 引物 5’ 端引入 15-25 bp 的线性化载体末端同源序列，使得插入片段 PCR 产物 ... WebUniversal one step cloning kit, a one step cloning kit, is developed by seamless cloning technology based on homologous recombinase. Compared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one … fn/a https://theyellowloft.com

分子克隆技术-一步法克隆-丁香实验

Web13 de abr. de 2024 · 高保真聚合酶（Hieff Canace Plus High-Fidelity DNA Polymerase，货号 10153ES60） 一步法克隆试剂盒（Hieff Clone ® Plus One Step Cloning Kit 一步 … Web20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … Web9 de dez. de 2024 · Then, the upstream (before the start codon of the gene) and downstream (after the stop codon of the gene) fragment of each gene and the enzymatically digested vector were mixed together by seamless cloning with the Hieff Clone® Plus Multi One Step Cloning Kit (YEASEN, Shanghai, China), to get the plasmids pKC1139S … fnaa free play

ClonExpress II One Step Cloning Kit_诺唯赞—科技成就健康生活

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Hieff clone plus multi one step cloning kit

TA Cloning Kits Thermo Fisher Scientific - US

Web12 de ago. de 2024 · The full-length cDNA of OsEBF1 (LOC_Os06g40360) was amplified with KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) using the primers EBF1OE-attB-F and EBF1OE-attB-R, and cloned into the p1301-35SNos vector using a Hieff-Clone™ Plus Multi One Step Cloning Kit (Yeasen, Beijing, China). Web17 de mar. de 2024 · Multi-dimensional proteomics outlines systematic cellular response to rapamycin ... Hieff Clone Plus Multi One Step Cloning Kit: Yeasen: Cat#10912ES10: PolyJet reagent: SignaGen: Cat#SL100688: PCR purification kit: QIAGEN: Cat#EN0531: SYBR Green Supermix: Thermo Fisher Scientific: Cat#34364344:

Hieff clone plus multi one step cloning kit

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WebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. WebHieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可显著提高重 …

Web9 de dez. de 2024 · HR donor plasmids were constructed using the Hieff Clone Plus Multi One Step Cloning Kit (YEASEN, China). The G622A HR donor was constructed by ligating four fragments (a left arm, ... WebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite.

Web1 de jul. de 2024 · Secondly, fragments of EGFP coding sequence (amplified with EGFP-ORF-F and EGFP-ORF-R) and Pxvasa 3′UTR (amplified with vasa3′UTR-F and vasa3′UTR-R) were used to construct two transient expression vectors vasaP2k-EGFP-SV40 and vasaP1214-EGFP-vasa3′UTR using Hieff Clone Plus Multi One Step Cloning Kit … WebThe Hieff Clone™ Universal One Step Cloning Kit ensures efficient single- and multi-fragment cloning. A-B: Recombinant transformation plates. C: Electropherogram of …

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Web27 de abr. de 2024 · PCR of the coding sequence of Met or Gce from the pClone007-Met or pClone007-Gce vector was performed, and then we used a Hieff Clone Plus Multi One Step Cloning Kit (10912ES10, Yeasen) for assembly. Finally, we obtained the pUAST-Met-4 × FLAG-Avi-P2A-BirA ( UAS-Met-BirA ) or pUAST-Gce-3 × FLAG-Avi-P2A-BirA ( UAS … fn99 auto recyclersWebTA Cloning kits are available without competent cells (K2024-20 and K2024-40) or with Invitrogen One Shot INVαF’ Chemically Competent E. coli (K2000-01 and K2000-40), and One Shot TOP10F’ Chemically Competent E. coli (K2040-01 and K2040-40) in both 20 and 40-reaction pack sizes.For TA Cloning with the pCR II Vector (dual promoter) kits are … fna asc gl - home sharepoint.comWebBasic Steps of Cloning. There are many approaches available for cloning DNA, most of which follow this basic workflow: Isolation of target DNA - typically referred to as an … greens of merrill creekWebFigure 5. Single fragment cloning of 2290 bp was performed with the Hieff Clone™ Plus One Step Cloning Kit. A: line 1 is the linear vector, and line 2 is the target DNA … greens of mepal pricesWebHieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片段。 … greens of madison apartmentsWebToo lazy to do cloning work? Empower your laziness with our Pro Ligation-free Cloning Kit! This bestselling kit offers:1) One-step cloning - no reliance on r... greens of mortenhamsteadWebElisa Kit / ... Hieff Clone ® 多片段一 ... 10912ES10. 10 T. Hieff Mut TM 定点突变试剂盒. 11003ES10. 10 T . HB211123. 推荐商品 (10911ES) Hieff Clone ® Plus One Step … fna anesthesia